CO1 Amplicon Sequence Variants of bulk arthropod samples (mild lysis) collected with Malaise traps from the Insect Biome Atlas project in Madagascar

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Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Miraldo A, Sundh J, Iwaszkiewicz-Eggebrecht E, Granqvist E, Manoharan L, Łukasik P, Tack A J M, Andersson A F, Roslin T, Ronquist F (2025). CO1 Amplicon Sequence Variants of bulk arthropod samples (mild lysis) collected with Malaise traps from the Insect Biome Atlas project in Madagascar. Version 1.3. Swedish Museum of Natural History. Occurrence dataset. https://www.gbif.se/ipt/resource?r=iba_co1_lysate_2019_mg&v=1.3

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O editor e o detentor dos direitos deste trabalho é Swedish Museum of Natural History. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

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Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: bb3ce407-964b-411a-90ce-d42b394dd646.  Swedish Museum of Natural History publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por GBIF Sweden.

Palavras-chave

Metadata; Observation

Contatos

Cobertura Geográfica

Madagascar

Cobertura Taxonômica

Insects

Cobertura Temporal

Dados Sobre o Projeto

The Insect Biome Atlas (IBA) is a large-scale insect project aimed at charting the insect faunas of Sweden and Madagascar and their associated microbiomes using high-throughput DNA sequencing of Malaise trap and soil samples. The main aim is to describe the insect faunas of these two biologically and geologically very different countries in unprecedented detail. The Swedish insect fauna consists of postglacial immigrants and is one of the best studied in the world. Madagascar is the world’s most important biodiversity hotspot, with a long period of evolution in isolation and where many species are critically endangered if not already extinct. During the field phase in 2019-2020, weekly samples were collected in both countries for a 12-month period. Insects were collected using Malaise traps: 203 traps in Sweden and 50 in Madagascar. Several other types of samples and ecological measurements were also collected at all trap sites to gather a full understanding of the ecological roles of the organisms that comprise the insect biomes and associated microbiomes. In total, IBA collected ca 8,000 insect community samples. The identification of all insects in the material and the organisms they interact with, such as pathogens as well as symbiotic fungi and bacteria, was achieved using metabarcoding and high-throughput DNA sequencing. In Sweden the Malaise traps were managed by over 150 volunteers, which makes this project one of the largest citizen science projects to take place in Scandinavia. The Swedish inventory was implemented in collaboration with Station Linné and the Malagasy inventory in collaboration with Madagascar Biodiversity Centre, both very active entomological research stations.

O pessoal envolvido no projeto:

Métodos de Amostragem

Malaise trap samples were collected at all sampling locations of the Insect Biome Atlas project in Madagascar (50 Malaise traps in total). Arthropods were collected in individually barcoded bottles pre-filled with 400 mL of 95% ethanol. Malaise traps were active between August 2019 and July 2020. Samples were collected every week . Please note that the results presented here correspond only to samples processed using the FAVIS protocol (mild lysis). See 'Malaise trap samples' and 'Mild lysis' extraction in https://doi.org/10.1038/s41597-025-05151-0.

Descrição dos passos do método:

1. See 'Malaise trap samples' and 'Mild lysis' extraction in https://doi.org/10.1038/s41597-025-05151-0.

Citações bibliográficas

1. Miraldo, A., Sundh, J., Iwaszkiewicz-Eggebrecht, E., Buczek, M., Goodsell, R., Johansson, H., Fisher, B. L., Raharinjanahary, D., Rajoelison, E. T., Ranaivo, C., Randrianandrasana, C., Rafanomezantsoa, J.-J., Manoharan, L., Granqvist, E., van Dijk, L. J. A., Alberg, L., Åhlén, D., Aspebo, M., Åström, S., Bellviken, A., Bergman, P.-E., Björklund, S., Björkman, M. P., Deng, J., Desborough, L., Dolff, E., Eliasson, A., Elmquist, H., Emanuelsson, H., Erixon, R., Fahlen, L., Frogner, C., Fürst, P., Grabs, A., Grudd, H., Guasconi, D., Gunnarsson, M., Häggqvist, S., Hed, A., Hörnström, E., Johansson, H., Jönsson, A., Kanerot, S., Karlsson, A., Karlsson, D., Klinth, M., Kraft, T., Lahti, R., Larsson, M., Lernefalk, H., Lestander, Y., Lindholm, L.-T., Lindholm, M., Ljung, U., Ljung, K., Lundberg, J., Lundin, E., Malmenius, M., Marquina, D., Martinelli, J., Mertz, L., Nilsson, J., Patchett, A., Persson, N., Persson, J., Prus-Frankowska, M., Regazzoni, E., Rosander, K.-G., Rydgård, M., Sandblom, C., Skord, J., Stålhandske, T., Svensson, F., Szpryngiel, S., Tajani, K., Tyboni, M., Ugarph, C., Vestermark, L., Vilhelmsson, J., Wahlgren, N., Wass, A., Wetterstrand, P., Łukasik, P., Tack, A. J. M., Andersson, A. F., Roslin, T., & Ronquist, F. (2024). Data of the Insect Biome Atlas: a metabarcoding survey of the terrestrial arthropods of Sweden and Madagascar. Sci Data 12, 835 (2025). https://doi.org/10.1038/s41597-025-05151-0

Metadados Adicionais