CO1 Amplicon Sequence Variants of bulk arthropod samples (mild lysis) collected with Malaise traps from the Insect Biome Atlas project in Sweden (2021)

Registro biológico
Última versión publicado por Swedish Museum of Natural History el oct 13, 2026 Swedish Museum of Natural History
Fecha de publicación:
13 de octubre de 2026
Licencia:
CC-BY 4.0

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Descripción

Amplicon Sequence Variants of 418bp of CO1 from mild lysed arthropod community samples collected with Malaise traps in 2021 from the Insect Biome Atlas project in Sweden, as described in the IBA data paper.This dataset was published via the SBDI ASV portal.The current version was reprocessed from Processed ASV data from the Insect Biome Atlas Project v4: ASVs re-clustered with HAPP using an updated COI reference database (COIDB v5), noise removal with the NEEAT filter, and additional clean-up.Occurrence data were compiled from the following IBA Figshare items:Processed ASV data from the Insect Biome Atlas Project v4:cleaned_noise_filtered_cluster_taxonomy_SE.tsv.gzcleaned_noise_filtered_cluster_counts_SE.tsv.gzspikeins_tax_SE.tsv.gzremoved_control_tax_SE.tsv.gzAmplicon sequence variants from the Insect Biome Atlas project v7:CO1_asv_counts_SE.tsv.gzCO1_asv_seqs_SE.fasta.gzCO1_sequencing_metadata_SE.tsvsamples_metadata_malaise_SE.tsvsites_metadata_SE.tsv

Registros

Los datos en este recurso de registros biológicos han sido publicados como Archivo Darwin Core(DwC-A), el cual es un formato estándar para compartir datos de biodiversidad como un conjunto de una o más tablas de datos. La tabla de datos del core contiene 970 registros.

también existen 2 tablas de datos de extensiones. Un registro en una extensión provee información adicional sobre un registro en el core. El número de registros en cada tabla de datos de la extensión se ilustra a continuación.

Occurrence (core)
970
ExtendedMeasurementOrFact 
1940
dnaDerivedData 
970

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Palabras clave

Occurrence

Contactos

Andreia Miraldo
  • Originador
  • Researcher
Swedish Museum of Natural History
Stockholm
SE
John Sundh
  • Originador
Stockholm University
Stockholm
SE
Elzbieta Iwaszkiewicz-Eggebrecht
  • Originador
Swedish Museum of Natural History
Stockholm
SE
Emma Granqvist
  • Originador
Swedish Museum of Natural History
Stockholm
SE
Lokeshwaran Manoharan
  • Originador
Lund University
Lund
SE
Piotr Łukasik
  • Originador
Jagiellonian University
Kraków
PL
Ayco J. M. Tack
  • Originador
Stockholm University,
Stockholm
SE
Anders F. Andersson
  • Originador
KTH Royal Institute of Technology
Stockholm
SE
Tomas Roslin
  • Originador
Swedish University of Agricultural Sciences (SLU)
Uppsala
SE
Fredrik Ronquist
  • Originador ●
  • Punto De Contacto
  • Professor
Swedish Museum of Natural History
SE

Cobertura geográfica

Sweden

Coordenadas límite Latitud Mínima Longitud Mínima [56,025, 13,607], Latitud Máxima Longitud Máxima [56,931, 16,83]

Cobertura taxonómica

Arthropods

Filo Arthropoda, Nematoda, Oomycota
Class Arachnida, Chromadorea, Collembola, Insecta, Peronosporea
Orden Araneae, Coleoptera, Dermaptera, Diptera, Entomobryomorpha, Hemiptera, Hymenoptera, Lepidoptera, Mesostigmata, Neuroptera, Neuropterida, Opiliones, Orthoptera, Peronosporales, Poduromorpha, Psocodea, Rhabditida, Sarcoptiformes, Symphypleona, Thysanoptera, Trichoptera, Trombidiformes

Cobertura temporal

Fecha Inicial / Fecha Final 2021-05-22 / 2021-05-30

Datos del proyecto

The Insect Biome Atlas (IBA) is a large-scale insect project aimed at charting the insect faunas of Sweden and Madagascar and their associated microbiomes using high-throughput DNA sequencing of Malaise trap and soil samples. The main aim is to describe the insect faunas of these two biologically and geologically very different countries in unprecedented detail. The Swedish insect fauna consists of postglacial immigrants and is one of the best studied in the world. Madagascar is the world’s most important biodiversity hotspot, with a long period of evolution in isolation and where many species are critically endangered if not already extinct. During the field phase in 2019-2020, weekly samples were collected in both countries for a 12-month period. Insects were collected using Malaise traps: 203 traps in Sweden and 50 in Madagascar. Several other types of samples and ecological measurements were also collected at all trap sites to gather a full understanding of the ecological roles of the organisms that comprise the insect biomes and associated microbiomes. In total, IBA collected ca 8,000 insect community samples. The identification of all insects in the material and the organisms they interact with, such as pathogens as well as symbiotic fungi and bacteria, was achieved using metabarcoding and high-throughput DNA sequencing. In Sweden the Malaise traps were managed by over 150 volunteers, which makes this project one of the largest citizen science projects to take place in Scandinavia. The Swedish inventory was implemented in collaboration with Station Linné and the Malagasy inventory in collaboration with Madagascar Biodiversity Centre, both very active entomological research stations.

Título Insect Biome Atlas: a metabarcoding survey of the terrestrial arthropods of Sweden and Madagascar
Identificador IBA-NRM-KAW-2017-0088
Fuentes de Financiación This work was supported by the Knut and Alice Wallenberg Foundation (grant KAW 2017.088 to FR), the Swedish Research Council (grant 2018-04620 to FR, grant 2021-03784 to AJMT), the Polish National Agency for Academic Exchange (grant PPN/PPO/2018/1/00015 to PL), the Polish National Science Centre (grant 2018/31/B/NZ8/01158 to PL), the European Research Council (Synergy Grant 856506 to TR), the Swedish University of Agricultural Sciences (Career Support grant to TR), the Science for Life Laboratory (SciLifeLab) & Wallenberg Data Driven Life Science Program funded by Knut and Alice Wallenberg Foundation (grants: KAW 2020.0239 and KAW 2017.0003), the National Bioinformatics Infrastructure Sweden (NBIS) at SciLifeLab, the Abisko Scientific Research Station and the Swedish Infrastructure for Ecosystem Science (Swedish Research Council grant no. 4.3-2021-00164) and the strategic research area BECC - Biodiversity and Ecosystem services in a Changing Climate. The computations and data handling were enabled by resources in projects SNIC 2020/15-307, SNIC 2022/5-211, NAISS 2023/5-209, and NAISS 2024/5-207 (compute) and SNIC 2020/16-248 (storage), provided by the National Academic Infrastructure for Supercomputing in Sweden (NAISS) and the Swedish National Infrastructure for Computing (SNIC) at Uppsala Multidisciplinary Center for Advanced Computational Science (UPPMAX), partially funded by the Swedish Research Council (grant 2022-06725 and 2018-05973). The authors also acknowledge support from the National Genomics Infrastructure in Stockholm. For the work carried out in Madagascar, the authors acknowledge support from the Madagascar National Parks, Fanamby, Asity, Groupe d'Etudes et de Recherches sur les Primates (GERP), Centre National de Formation, d’Etudes et de Recherches en Environnement et Foresterie (CNFEREF), Missouri Botanical Garden (MBG), Ambatovy, Ministère de l'Environnement et du Développement Durable, Parc Botanique et Zoologique de Tsimbazaza and Laboratoires des Radioisotopes.

Personas asociadas al proyecto:

Fredrik Ronquist

Métodos de muestreo

Four Malaise trap samples collected in May 2021 (2021-05-22 to 2021-05-30) at four Insect Biome Atlas trap locations in southern Sweden (traps THBEZ5, TIRXWF, TM9NNJ, TMXZCE), processed using the FAVIS protocol (mild lysis). See 'Malaise trap samples' and 'Mild lysis' extraction in https://doi.org/10.1038/s41597-025-05151-0.

Área de Estudio Malaise trap locations of the Insect Biome Atlas project in Sweden.

Descripción de la metodología paso a paso:

  1. See 'Malaise trap samples' and 'Mild lysis' extraction in https://doi.org/10.1038/s41597-025-05151-0.

Referencias bibliográficas

  1. Miraldo, A., Sundh, J., Iwaszkiewicz-Eggebrecht, E., Buczek, M., Goodsell, R., Johansson, H., Fisher, B. L., Raharinjanahary, D., Rajoelison, E. T., Ranaivo, C., Randrianandrasana, C., Rafanomezantsoa, J.-J., Manoharan, L., Granqvist, E., van Dijk, L. J. A., Alberg, L., Åhlén, D., Aspebo, M., Åström, S., Bellviken, A., Bergman, P.-E., Björklund, S., Björkman, M. P., Deng, J., Desborough, L., Dolff, E., Eliasson, A., Elmquist, H., Emanuelsson, H., Erixon, R., Fahlen, L., Frogner, C., Fürst, P., Grabs, A., Grudd, H., Guasconi, D., Gunnarsson, M., Häggqvist, S., Hed, A., Hörnström, E., Johansson, H., Jönsson, A., Kanerot, S., Karlsson, A., Karlsson, D., Klinth, M., Kraft, T., Lahti, R., Larsson, M., Lernefalk, H., Lestander, Y., Lindholm, L.-T., Lindholm, M., Ljung, U., Ljung, K., Lundberg, J., Lundin, E., Malmenius, M., Marquina, D., Martinelli, J., Mertz, L., Nilsson, J., Patchett, A., Persson, N., Persson, J., Prus-Frankowska, M., Regazzoni, E., Rosander, K.-G., Rydgård, M., Sandblom, C., Skord, J., Stålhandske, T., Svensson, F., Szpryngiel, S., Tajani, K., Tyboni, M., Ugarph, C., Vestermark, L., Vilhelmsson, J., Wahlgren, N., Wass, A., Wetterstrand, P., Łukasik, P., Tack, A. J. M., Andersson, A. F., Roslin, T., & Ronquist, F. (2024). Data of the Insect Biome Atlas: a metabarcoding survey of the terrestrial arthropods of Sweden and Madagascar. Sci Data 12, 835 (2025). https://doi.org/10.1038/s41597-025-05151-0

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