Spatiotemporal Baltic Sea area 16S metabarcoding from three projects performed in 2015-2017 (+storage test 2019)

Última versión publicado por KTH Royal Institute of Technology el abr 23, 2025 KTH Royal Institute of Technology
Fecha de publicación:
23 de abril de 2025
Licencia:
CC-BY 4.0

Descargue la última versión de los metadatos como EML o RTF:

Metadatos como un archivo EML descargar en Inglés (11 KB)
Metadatos como un archivo RTF descargar en Inglés (8 KB)

Descripción

This dataset was published via the SBDI ASV portal. Occurrence data will be added following manuscript publication.16S (V3-V4) metabarcoding results shared here come from the following sampling efforts:1. Bi-weekly sampling along the Swedish marine monitoring program across twelve locations in Baltic Proper, Kattegat, and Skagerrak. For all the locations, sampling was performed between February 2016 and March 2017. Additionally, one station (Släggö, Skagerrak) was sampled from August 2015.2. Weekly sampling at multiple depths (5, 10, and 15 meters in most cases) at Tångesund, Sweden (Skagerrak), performed in 2016 between August 22nd and October 10th.3. Three longitudinal transects in Skagerrak, performed in 2016 on August 18th, 4. Replicate samples collected at Släggö marine station (Skagerrak) on the same date (August 20th 2019) and stored as filters before DNA extraction for 1 week, 1 month, 3 months, or 6 months, in either -20⁰C or -80⁰C.The extracted DNA was stored at -20⁰C until mid-2023 when the amplicon sequencing was performed. All the contextual data has been obtained from the SharkWeb portal maintained by the Swedish Meteorological and Hydrological Institute.

Versiones

La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.

¿Cómo referenciar?

Los usuarios deben citar este trabajo de la siguiente manera:

Jurdzinski K (2025). Spatiotemporal Baltic Sea area 16S metabarcoding from three projects performed in 2015-2017 (+storage test 2019). Version 1.1. KTH Royal Institute of Technology. Metadata dataset. https://www.biorxiv.org/content/10.1101/2024.08.14.607742

Derechos

Los usuarios deben respetar los siguientes derechos de uso:

El publicador y propietario de los derechos de este trabajo es KTH Royal Institute of Technology. Esta obra está bajo una licencia Creative Commons de Atribución/Reconocimiento (CC-BY 4.0).

Registro GBIF

Este recurso ha sido registrado en GBIF con el siguiente UUID: fbf49d0a-df4c-4b89-ba71-143bf6430a91.  KTH Royal Institute of Technology publica este recurso y está registrado en GBIF como un publicador de datos avalado por GBIF Sweden.

Palabras clave

Metadata

Contactos

Krzysztof Jurdzinski
  • Proveedor De Los Metadatos
  • Originador
  • Punto De Contacto
KTH Royal Institute of Technology
Anders F Andersson
  • Punto De Contacto
  • Professor
KTH Royal Institute of Technology
Bengt Karlson
  • Punto De Contacto
  • Senior researcher
Swedish Meteorological and Hydrological Institute

Cobertura geográfica

The Baltic Sea area - the Baltic Sea and the Kattegat/Skagerrak.

Coordenadas límite Latitud Mínima Longitud Mínima [52, 5], Latitud Máxima Longitud Máxima [66, 25]

Cobertura temporal

Fecha Inicial / Fecha Final 2015-07-08 / 2019-10-07

Datos del proyecto

16S (V3-V4) metabarcoding results shared here come from the following sampling efforts: 1. Bi-weekly sampling along the Swedish marine monitoring program across twelve locations in Baltic Proper, Kattegat, and Skagerrak. For all the locations, sampling was performed between February 2016 and March 2017. Additionally, one station (Släggö, Skagerrak) was sampled from August 2015. 2. Weekly sampling at multiple depths (5, 10, and 15 meters in most cases) at Tångesund, Sweden (Skagerrak), performed in 2016 between August 22nd and October 10th. 3. Three longitudinal transects in Skagerrak, performed in 2016 on August 18th, 4. Replicate samples collected at Släggö marine station (Skagerrak) on the same date (August 20th 2019) and stored as filters before DNA extraction for 1 week, 1 month, 3 months, or 6 months, in either -20⁰C or -80⁰C. The extracted DNA was stored at -20⁰C until mid-2023 when the amplicon sequencing was performed. All the contextual data has been obtained from the SharkWeb portal maintained by the Swedish Meteorological and Hydrological Institute.

Título Bi-weekly monitoring 2015-2017; Weekly sampling at multiple depths; Three longitudinal transects in Skagerrak; Storage test
Fuentes de Financiación The Swedish Agency for Marine and Water Management and the Swedish Environmental Protection Agency under grant number NV-03728-17. AFA was additionally funded by a research grant (2021-05563). The Swedish Research Council FORMAS (grant number 214-2013-1449). The EU Horizon 2020 project JERICO-NEXT under grant agreement No 654410

Personas asociadas al proyecto:

Anders F Andersson
Bengt Karlson
Krzysztof Jurdzinski

Métodos de muestreo

Mainly hose sampling from aboard a research cruise vessel. Tangesund samples were collected with a bottle, thus they come from a particular depth and not a range of depths.

Área de Estudio The Baltic Sea Area (The Baltic Sea, Kattegat, Skagerrak); January 2016 to March 2017 with a few extra samples from 2015 and 2019

Descripción de la metodología paso a paso:

  1. 1. Water collection 2. Filtering 3. DNA extraction 4. Illumina NextSeq sequencing 5. Data processing

Referencias bibliográficas

  1. Distinct bacterial and protist plankton diversity dynamics uncovered through DNA-based monitoring in the Baltic Sea area Krzysztof T Jurdzinski, Meike AC Latz, Anders Torstensson, Sonia Brugel, Mikael Hedblom, Yue O O Hu, Markus Lindh, Agneta Andersson, Bengt Karlson, Anders F Andersson https://www.biorxiv.org/content/10.1101/2024.08.14.607742

Metadatos adicionales