Spatiotemporal Baltic Sea area 16S metabarcoding from three projects performed in 2015-2017 (+storage test 2019)

Métadonnées uniquement
Dernière version Publié par KTH Royal Institute of Technology le avr. 23, 2025 KTH Royal Institute of Technology
Date de publication:
23 avril 2025
Licence:
CC-BY 4.0

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Description

This dataset was published via the SBDI ASV portal. Occurrence data will be added following manuscript publication.16S (V3-V4) metabarcoding results shared here come from the following sampling efforts:1. Bi-weekly sampling along the Swedish marine monitoring program across twelve locations in Baltic Proper, Kattegat, and Skagerrak. For all the locations, sampling was performed between February 2016 and March 2017. Additionally, one station (Släggö, Skagerrak) was sampled from August 2015.2. Weekly sampling at multiple depths (5, 10, and 15 meters in most cases) at Tångesund, Sweden (Skagerrak), performed in 2016 between August 22nd and October 10th.3. Three longitudinal transects in Skagerrak, performed in 2016 on August 18th, 4. Replicate samples collected at Släggö marine station (Skagerrak) on the same date (August 20th 2019) and stored as filters before DNA extraction for 1 week, 1 month, 3 months, or 6 months, in either -20⁰C or -80⁰C.The extracted DNA was stored at -20⁰C until mid-2023 when the amplicon sequencing was performed. All the contextual data has been obtained from the SharkWeb portal maintained by the Swedish Meteorological and Hydrological Institute.

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Comment citer

Les chercheurs doivent citer cette ressource comme suit:

Jurdzinski K (2025). Spatiotemporal Baltic Sea area 16S metabarcoding from three projects performed in 2015-2017 (+storage test 2019). Version 1.1. KTH Royal Institute of Technology. Metadata dataset. https://www.biorxiv.org/content/10.1101/2024.08.14.607742

Droits

Les chercheurs doivent respecter la déclaration de droits suivante:

L’éditeur et détenteur des droits de cette ressource est KTH Royal Institute of Technology. Ce travail est sous licence Creative Commons Attribution (CC-BY) 4.0.

Enregistrement GBIF

Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : fbf49d0a-df4c-4b89-ba71-143bf6430a91.  KTH Royal Institute of Technology publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du GBIF Sweden.

Mots-clé

Metadata

Contacts

Krzysztof Jurdzinski
  • Fournisseur Des Métadonnées ●
  • Créateur ●
  • Personne De Contact
KTH Royal Institute of Technology
Anders F Andersson
  • Personne De Contact
  • Professor
KTH Royal Institute of Technology
Bengt Karlson
  • Personne De Contact
  • Senior researcher
Swedish Meteorological and Hydrological Institute

Couverture géographique

The Baltic Sea area - the Baltic Sea and the Kattegat/Skagerrak.

Enveloppe géographique Sud Ouest [52, 5], Nord Est [66, 25]

Couverture temporelle

Date de début / Date de fin 2015-07-08 / 2019-10-07

Données sur le projet

16S (V3-V4) metabarcoding results shared here come from the following sampling efforts: 1. Bi-weekly sampling along the Swedish marine monitoring program across twelve locations in Baltic Proper, Kattegat, and Skagerrak. For all the locations, sampling was performed between February 2016 and March 2017. Additionally, one station (Släggö, Skagerrak) was sampled from August 2015. 2. Weekly sampling at multiple depths (5, 10, and 15 meters in most cases) at Tångesund, Sweden (Skagerrak), performed in 2016 between August 22nd and October 10th. 3. Three longitudinal transects in Skagerrak, performed in 2016 on August 18th, 4. Replicate samples collected at Släggö marine station (Skagerrak) on the same date (August 20th 2019) and stored as filters before DNA extraction for 1 week, 1 month, 3 months, or 6 months, in either -20⁰C or -80⁰C. The extracted DNA was stored at -20⁰C until mid-2023 when the amplicon sequencing was performed. All the contextual data has been obtained from the SharkWeb portal maintained by the Swedish Meteorological and Hydrological Institute.

Titre Bi-weekly monitoring 2015-2017; Weekly sampling at multiple depths; Three longitudinal transects in Skagerrak; Storage test
Financement The Swedish Agency for Marine and Water Management and the Swedish Environmental Protection Agency under grant number NV-03728-17. AFA was additionally funded by a research grant (2021-05563). The Swedish Research Council FORMAS (grant number 214-2013-1449). The EU Horizon 2020 project JERICO-NEXT under grant agreement No 654410

Les personnes impliquées dans le projet:

Anders F Andersson
  • Propriétaire
Bengt Karlson
  • Propriétaire
Krzysztof Jurdzinski
  • Créateur

Méthodes d'échantillonnage

Mainly hose sampling from aboard a research cruise vessel. Tangesund samples were collected with a bottle, thus they come from a particular depth and not a range of depths.

Etendue de l'étude The Baltic Sea Area (The Baltic Sea, Kattegat, Skagerrak); January 2016 to March 2017 with a few extra samples from 2015 and 2019

Description des étapes de la méthode:

  1. 1. Water collection 2. Filtering 3. DNA extraction 4. Illumina NextSeq sequencing 5. Data processing

Citations bibliographiques

  1. Distinct bacterial and protist plankton diversity dynamics uncovered through DNA-based monitoring in the Baltic Sea area Krzysztof T Jurdzinski, Meike AC Latz, Anders Torstensson, Sonia Brugel, Mikael Hedblom, Yue O O Hu, Markus Lindh, Agneta Andersson, Bengt Karlson, Anders F Andersson https://www.biorxiv.org/content/10.1101/2024.08.14.607742

Métadonnées additionnelles