Spatiotemporal Baltic Sea area 16S metabarcoding from three projects performed in 2015-2017 (+storage test 2019)

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Versão mais recente published by KTH Royal Institute of Technology on abr. 23, 2025 KTH Royal Institute of Technology
Publication date:
23 de abril de 2025
Licença:
CC-BY 4.0

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Descrição

This dataset was published via the SBDI ASV portal. Occurrence data will be added following manuscript publication.16S (V3-V4) metabarcoding results shared here come from the following sampling efforts:1. Bi-weekly sampling along the Swedish marine monitoring program across twelve locations in Baltic Proper, Kattegat, and Skagerrak. For all the locations, sampling was performed between February 2016 and March 2017. Additionally, one station (Släggö, Skagerrak) was sampled from August 2015.2. Weekly sampling at multiple depths (5, 10, and 15 meters in most cases) at Tångesund, Sweden (Skagerrak), performed in 2016 between August 22nd and October 10th.3. Three longitudinal transects in Skagerrak, performed in 2016 on August 18th, 4. Replicate samples collected at Släggö marine station (Skagerrak) on the same date (August 20th 2019) and stored as filters before DNA extraction for 1 week, 1 month, 3 months, or 6 months, in either -20⁰C or -80⁰C.The extracted DNA was stored at -20⁰C until mid-2023 when the amplicon sequencing was performed. All the contextual data has been obtained from the SharkWeb portal maintained by the Swedish Meteorological and Hydrological Institute.

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Pesquisadores deveriam citar esta obra da seguinte maneira:

Jurdzinski K (2025). Spatiotemporal Baltic Sea area 16S metabarcoding from three projects performed in 2015-2017 (+storage test 2019). Version 1.1. KTH Royal Institute of Technology. Metadata dataset. https://www.biorxiv.org/content/10.1101/2024.08.14.607742

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O editor e o detentor dos direitos deste trabalho é KTH Royal Institute of Technology. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: fbf49d0a-df4c-4b89-ba71-143bf6430a91.  KTH Royal Institute of Technology publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por GBIF Sweden.

Palavras-chave

Metadata

Contatos

Krzysztof Jurdzinski
  • Provedor Dos Metadados ●
  • Originador ●
  • Ponto De Contato
KTH Royal Institute of Technology
Anders F Andersson
  • Ponto De Contato
  • Professor
KTH Royal Institute of Technology
Bengt Karlson
  • Ponto De Contato
  • Senior researcher
Swedish Meteorological and Hydrological Institute

Cobertura Geográfica

The Baltic Sea area - the Baltic Sea and the Kattegat/Skagerrak.

Coordenadas delimitadoras Sul Oeste [52, 5], Norte Leste [66, 25]

Cobertura Temporal

Data Inicial / Data final 2015-07-08 / 2019-10-07

Dados Sobre o Projeto

16S (V3-V4) metabarcoding results shared here come from the following sampling efforts: 1. Bi-weekly sampling along the Swedish marine monitoring program across twelve locations in Baltic Proper, Kattegat, and Skagerrak. For all the locations, sampling was performed between February 2016 and March 2017. Additionally, one station (Släggö, Skagerrak) was sampled from August 2015. 2. Weekly sampling at multiple depths (5, 10, and 15 meters in most cases) at Tångesund, Sweden (Skagerrak), performed in 2016 between August 22nd and October 10th. 3. Three longitudinal transects in Skagerrak, performed in 2016 on August 18th, 4. Replicate samples collected at Släggö marine station (Skagerrak) on the same date (August 20th 2019) and stored as filters before DNA extraction for 1 week, 1 month, 3 months, or 6 months, in either -20⁰C or -80⁰C. The extracted DNA was stored at -20⁰C until mid-2023 when the amplicon sequencing was performed. All the contextual data has been obtained from the SharkWeb portal maintained by the Swedish Meteorological and Hydrological Institute.

Título Bi-weekly monitoring 2015-2017; Weekly sampling at multiple depths; Three longitudinal transects in Skagerrak; Storage test
Financiamento The Swedish Agency for Marine and Water Management and the Swedish Environmental Protection Agency under grant number NV-03728-17. AFA was additionally funded by a research grant (2021-05563). The Swedish Research Council FORMAS (grant number 214-2013-1449). The EU Horizon 2020 project JERICO-NEXT under grant agreement No 654410

O pessoal envolvido no projeto:

Anders F Andersson
  • Proprietário
Bengt Karlson
  • Proprietário
Krzysztof Jurdzinski
  • Originador

Métodos de Amostragem

Mainly hose sampling from aboard a research cruise vessel. Tangesund samples were collected with a bottle, thus they come from a particular depth and not a range of depths.

Área de Estudo The Baltic Sea Area (The Baltic Sea, Kattegat, Skagerrak); January 2016 to March 2017 with a few extra samples from 2015 and 2019

Descrição dos passos do método:

  1. 1. Water collection 2. Filtering 3. DNA extraction 4. Illumina NextSeq sequencing 5. Data processing

Citações bibliográficas

  1. Distinct bacterial and protist plankton diversity dynamics uncovered through DNA-based monitoring in the Baltic Sea area Krzysztof T Jurdzinski, Meike AC Latz, Anders Torstensson, Sonia Brugel, Mikael Hedblom, Yue O O Hu, Markus Lindh, Agneta Andersson, Bengt Karlson, Anders F Andersson https://www.biorxiv.org/content/10.1101/2024.08.14.607742

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