Spatiotemporal Baltic Sea area 18S metabarcoding from three projects performed in 2015-2017 (+storage test 2019)

Latest version published by KTH Royal Institute of Technology on Apr 23, 2025 KTH Royal Institute of Technology
Publication date:
23 April 2025
License:
CC-BY 4.0

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Description

This dataset was published via the SBDI ASV portal. Occurrence data will be added following manuscript publication.18S (V4) metabarcoding results shared here come from the following sampling efforts:1. Bi-weekly sampling along the Swedish marine monitoring program across twelve locations in Baltic Proper, Kattegat, and Skagerrak. For all the locations, sampling was performed between February 2016 and March 2017. Additionally, one station (Släggö, Skagerrak) was sampled from August 2015.2. Weekly sampling at multiple depths (5, 10, and 15 meters in most cases) at Tångesund, Sweden (Skagerrak), performed in 2016 between August 22nd and October 10th.3. Three longitudinal transects in Skagerrak, performed in 2016 on August 18th, 4. Replicate samples collected at Släggö marine station (Skagerrak) on the same date (August 20th 2019) and stored as filters before DNA extraction for 1 week, 1 month, 3 months, or 6 months, in either -20⁰C or -80⁰C.The extracted DNA was stored at -20⁰C until mid-2023 when the amplicon sequencing was performed. All the contextual data has been obtained from the SharkWeb portal maintained by the Swedish Meteorological and Hydrological Institute.

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How to cite

Researchers should cite this work as follows:

Jurdzinski K (2025). Spatiotemporal Baltic Sea area 18S metabarcoding from three projects performed in 2015-2017 (+storage test 2019). Version 1.0. KTH Royal Institute of Technology. Metadata dataset. https://www.biorxiv.org/content/10.1101/2024.08.14.607742

Rights

Researchers should respect the following rights statement:

The publisher and rights holder of this work is KTH Royal Institute of Technology. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 9a228813-6288-4aba-a8f8-480e425b5f62.  KTH Royal Institute of Technology publishes this resource, and is itself registered in GBIF as a data publisher endorsed by GBIF Sweden.

Keywords

Metadata

Contacts

Krzysztof Jurdzinski
  • Metadata Provider
  • Originator
  • Point Of Contact
KTH Royal Institute of Technology
Anders F Andersson
  • Point Of Contact
  • Professor
KTH Royal Institute of Technology
Bengt Karlson
  • Point Of Contact
  • Senior researcher
Swedish Meteorological and Hydrological Institute

Geographic Coverage

The Baltic Sea area - the Baltic Sea and the Kattegat/Skagerrak.

Bounding Coordinates South West [52, 5], North East [66, 25]

Temporal Coverage

Start Date / End Date 2015-07-08 / 2019-10-07

Project Data

18S (V4) metabarcoding results shared here come from the following sampling efforts: 1. Bi-weekly sampling along the Swedish marine monitoring program across twelve locations in Baltic Proper, Kattegat, and Skagerrak. For all the locations, sampling was performed between February 2016 and March 2017. Additionally, one station (Släggö, Skagerrak) was sampled from August 2015. 2. Weekly sampling at multiple depths (5, 10, and 15 meters in most cases) at Tångesund, Sweden (Skagerrak), performed in 2016 between August 22nd and October 10th. 3. Three longitudinal transects in Skagerrak, performed in 2016 on August 18th, 4. Replicate samples collected at Släggö marine station (Skagerrak) on the same date (August 20th 2019) and stored as filters before DNA extraction for 1 week, 1 month, 3 months, or 6 months, in either -20⁰C or -80⁰C. The extracted DNA was stored at -20⁰C until mid-2023 when the amplicon sequencing was performed. All the contextual data has been obtained from the SharkWeb portal maintained by the Swedish Meteorological and Hydrological Institute.

Title Bi-weekly monitoring 2015-2017; Weekly sampling at multiple depths; Three longitudinal transects in Skagerrak; Storage test
Funding The Swedish Agency for Marine and Water Management and the Swedish Environmental Protection Agency under grant number NV-03728-17. AFA was additionally funded by a research grant (2021-05563). The Swedish Research Council FORMAS (grant number 214-2013-1449). The EU Horizon 2020 project JERICO-NEXT under grant agreement No 654410

The personnel involved in the project:

Sampling Methods

Mainly hose sampling from aboard a research cruise vessel. Tangesund samples were collected with a bottle, thus they come from a particular depth and not a range of depths.

Study Extent The Baltic Sea Area (The Baltic Sea, Kattegat, Skagerrak); January 2016 to March 2017 with a few extra samples from 2015 and 2019

Method step description:

  1. 1. Water collection 2. Filtering 3. DNA extraction 4. Illumina NextSeq sequencing 5. Data processing

Bibliographic Citations

  1. Distinct bacterial and protist plankton diversity dynamics uncovered through DNA-based monitoring in the Baltic Sea area Krzysztof T Jurdzinski, Meike AC Latz, Anders Torstensson, Sonia Brugel, Mikael Hedblom, Yue O O Hu, Markus Lindh, Agneta Andersson, Bengt Karlson, Anders F Andersson https://www.biorxiv.org/content/10.1101/2024.08.14.607742

Additional Metadata