Spatiotemporal Baltic Sea area 18S metabarcoding from three projects performed in 2015-2017 (+storage test 2019)

Последняя версия опубликовано KTH Royal Institute of Technology апр. 23, 2025 KTH Royal Institute of Technology
Дата публикации:
23 апреля 2025 г.
Опубликовано:
KTH Royal Institute of Technology
Лицензия:
CC-BY 4.0

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Описание

This dataset was published via the SBDI ASV portal. Occurrence data will be added following manuscript publication.18S (V4) metabarcoding results shared here come from the following sampling efforts:1. Bi-weekly sampling along the Swedish marine monitoring program across twelve locations in Baltic Proper, Kattegat, and Skagerrak. For all the locations, sampling was performed between February 2016 and March 2017. Additionally, one station (Släggö, Skagerrak) was sampled from August 2015.2. Weekly sampling at multiple depths (5, 10, and 15 meters in most cases) at Tångesund, Sweden (Skagerrak), performed in 2016 between August 22nd and October 10th.3. Three longitudinal transects in Skagerrak, performed in 2016 on August 18th, 4. Replicate samples collected at Släggö marine station (Skagerrak) on the same date (August 20th 2019) and stored as filters before DNA extraction for 1 week, 1 month, 3 months, or 6 months, in either -20⁰C or -80⁰C.The extracted DNA was stored at -20⁰C until mid-2023 when the amplicon sequencing was performed. All the contextual data has been obtained from the SharkWeb portal maintained by the Swedish Meteorological and Hydrological Institute.

Версии

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Как оформить ссылку

Исследователи должны дать ссылку на эту работу следующим образом:

Jurdzinski K (2025). Spatiotemporal Baltic Sea area 18S metabarcoding from three projects performed in 2015-2017 (+storage test 2019). Version 1.0. KTH Royal Institute of Technology. Metadata dataset. https://www.biorxiv.org/content/10.1101/2024.08.14.607742

Права

Исследователи должны соблюдать следующие права:

Публикующей организацией и владельцем прав на данную работу является KTH Royal Institute of Technology. Эта работа находится под лицензией Creative Commons Attribution (CC-BY 4.0).

Регистрация в GBIF

Этот ресурс был зарегистрирован в GBIF, ему был присвоен следующий UUID: 9a228813-6288-4aba-a8f8-480e425b5f62.  KTH Royal Institute of Technology отвечает за публикацию этого ресурса, и зарегистрирован в GBIF как издатель данных при оподдержке GBIF Sweden.

Ключевые слова

Metadata

Контакты

Krzysztof Jurdzinski
  • Metadata Provider
  • Originator
  • Point Of Contact
KTH Royal Institute of Technology
Anders F Andersson
  • Point Of Contact
  • Professor
KTH Royal Institute of Technology
Bengt Karlson
  • Point Of Contact
  • Senior researcher
Swedish Meteorological and Hydrological Institute

Географический охват

The Baltic Sea area - the Baltic Sea and the Kattegat/Skagerrak.

Ограничивающие координаты Юг Запад [52, 5], Север Восток [66, 25]

Временной охват

Дата начала / Дата окончания 2015-07-08 / 2019-10-07

Данные проекта

18S (V4) metabarcoding results shared here come from the following sampling efforts: 1. Bi-weekly sampling along the Swedish marine monitoring program across twelve locations in Baltic Proper, Kattegat, and Skagerrak. For all the locations, sampling was performed between February 2016 and March 2017. Additionally, one station (Släggö, Skagerrak) was sampled from August 2015. 2. Weekly sampling at multiple depths (5, 10, and 15 meters in most cases) at Tångesund, Sweden (Skagerrak), performed in 2016 between August 22nd and October 10th. 3. Three longitudinal transects in Skagerrak, performed in 2016 on August 18th, 4. Replicate samples collected at Släggö marine station (Skagerrak) on the same date (August 20th 2019) and stored as filters before DNA extraction for 1 week, 1 month, 3 months, or 6 months, in either -20⁰C or -80⁰C. The extracted DNA was stored at -20⁰C until mid-2023 when the amplicon sequencing was performed. All the contextual data has been obtained from the SharkWeb portal maintained by the Swedish Meteorological and Hydrological Institute.

Название Bi-weekly monitoring 2015-2017; Weekly sampling at multiple depths; Three longitudinal transects in Skagerrak; Storage test
Финансирование The Swedish Agency for Marine and Water Management and the Swedish Environmental Protection Agency under grant number NV-03728-17. AFA was additionally funded by a research grant (2021-05563). The Swedish Research Council FORMAS (grant number 214-2013-1449). The EU Horizon 2020 project JERICO-NEXT under grant agreement No 654410

Исполнители проекта:

Методы сбора

Mainly hose sampling from aboard a research cruise vessel. Tangesund samples were collected with a bottle, thus they come from a particular depth and not a range of depths.

Охват исследования The Baltic Sea Area (The Baltic Sea, Kattegat, Skagerrak); January 2016 to March 2017 with a few extra samples from 2015 and 2019

Описание этапа методики:

  1. 1. Water collection 2. Filtering 3. DNA extraction 4. Illumina NextSeq sequencing 5. Data processing

Библиографические ссылки

  1. Distinct bacterial and protist plankton diversity dynamics uncovered through DNA-based monitoring in the Baltic Sea area Krzysztof T Jurdzinski, Meike AC Latz, Anders Torstensson, Sonia Brugel, Mikael Hedblom, Yue O O Hu, Markus Lindh, Agneta Andersson, Bengt Karlson, Anders F Andersson https://www.biorxiv.org/content/10.1101/2024.08.14.607742

Дополнительные метаданные

Альтернативные идентификаторы https://www.gbif.se/ipt/resource?r=prjeb84926-18s