Descrição
Registros de Dados
Os dados deste recurso de ocorrência foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 9.922 registros.
Também existem 2 tabelas de dados de extensão. Um registro de extensão fornece informações adicionais sobre um registro do núcleo. O número de registros em cada tabela de dados de extensão é ilustrado abaixo.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
Versões
A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.
Como citar
Pesquisadores deveriam citar esta obra da seguinte maneira:
Johansson V (2026). Fungal ITS2 data from Pyroleae and Monotropa hypopitys germination study. Version 1.1. Stockholm University. Occurrence dataset. https://www.gbif.se/ipt/resource?r=pyrola-its2-2011-2012&v=1.1
Direitos
Pesquisadores devem respeitar a seguinte declaração de direitos:
O editor e o detentor dos direitos deste trabalho é Stockholm University. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF Registration
Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: d19074ea-f1cf-42ba-9109-e99a5e6a50a5. Stockholm University publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por GBIF Sweden.
Palavras-chave
Occurrence; ITS; metabarcoding; fungal communities; ectomycorrhizal fungi; mycoheterotrophy; seedling development; Pyroleae; Monotropa hypopitys; dust seeds; Sweden
Contatos
- Provedor Dos Metadados ●
- Originador ●
- Ponto De Contato
- GBIF Sweden Node Manager
- Box 50007
- +468-51954036
https://orcid.org/https://orcid.org/0000-0002-3028-9947
- Pesquisador Principal
- Autor
- Autor
- Autor
Cobertura Geográfica
Sampling was conducted on the island of Öland, Sweden, across multiple forest sites including coniferous and mixed forests.
| Coordenadas delimitadoras | Sul Oeste [56,438, 16,387], Norte Leste [57,353, 17,093] |
|---|
Cobertura Taxonômica
N/A
| Reino | Fungi |
|---|---|
| Filo | Mortierellomycota, Basidiobolomycota, Entorrhizomycota, Mucoromycota, Ascomycota, Zoopagomycota, Rozellomycota, Basidiomycota, Chytridiomycota |
| Class | Geminibasidiomycetes, Rhizophydiomycetes, Rozellomycota_cls_Incertae_sedis, Tremellomycetes, GS27, Atractiellomycetes, Spizellomycetes, Mortierellomycetes, Microbotryomycetes, Basidiobolomycota_cls_Incertae_sedis, Malasseziomycetes, Pezizomycotina_cls_Incertae_sedis, Dothideomycetes, Candelariomycetes, Eurotiomycetes, Entorrhizomycetes, Umbelopsidomycetes, Exobasidiomycetes, Sordariomycetes, Saccharomycetes, Lobulomycetes, Geoglossomycetes, Mucoromycetes, Pucciniomycetes, Rhizophlyctidomycetes, Archaeorhizomycetes, Arthoniomycetes, Agaricomycetes, Cystobasidiomycetes, Ascomycota_cls_Incertae_sedis, Rozellomycotina_cls_Incertae_sedis, Zoopagomycetes, Pezizomycetes, Basidiobolomycetes, Leotiomycetes, Orbiliomycetes |
| Ordem | Leucosporidiales, Pezizomycotina_ord_Incertae_sedis, Microthyriales, Filobasidiales, Myrmecridiales, Agaricomycetes_ord_Incertae_sedis, Eurotiomycetidae_ord_Incertae_sedis, Lichenostigmatales, Trichosphaeriales, Microbotryomycetes_ord_Incertae_sedis, Auriculariales, Sordariales, Leotiomycetes_ord_Incertae_sedis, Rhizophlyctidales, Cladosporiales, Mytilinidiales, Orbiliales, Onygenales, Verrucariales, Helicobasidiales, Exobasidiomycetes_ord_Incertae_sedis, Malasseziales, Tremellodendropsidales, Microascales, Cystofilobasidiales, Trichosporonales, Sebacinales, Dothideomycetes_ord_Incertae_sedis, Geminibasidiales, Corticiales, Geoglossales, Acrospermales, Mortierellales, Pseudodactylariales, Rhytismatales, Asterinales, Helotiales, Mucorales, Chaetosphaeriales, Agaricales, GS11, Cephalothecales, Atheliales, Trechisporales, Entorrhizales, Annulatascales, Rozellomycota_ord_Incertae_sedis, Basidiobolomycota_ord_Incertae_sedis, Candelariales, Pleosporales, Umbelopsidales, Pezizales, Glomerellales, Kriegeriales, Spizellomycetales, Polyporales, Sporidiobolales, Zoopagales, Mycosphaerellales, Eurotiales, Capnodiales, Patellariales, GS27_ord_Incertae_sedis, Hymenochaetales, Sclerococcales, Xylariales, Russulales, Hypocreales, Atractiellales, Botryosphaeriales, Tremellales, Chaetothyriales, Venturiales, Exobasidiales, Thelebolales, Tubeufiales, Magnaporthales, Thelephorales, Rhizophydiales, Leotiales, Gloniales, Lobulomycetales, Saccharomycetales, Boletales, Ascomycota_ord_Incertae_sedis, Archaeorhizomycetales, Coniochaetales, Cantharellales, Basidiobolales, Cystobasidiales, Phacidiales, Chaetomellales, Dothideales, Amphisphaeriales |
Métodos de Amostragem
Seeds of five plant species (Chimaphila umbellata, Moneses uniflora, Monotropa hypopitys, Pyrola chlorantha, and Pyrola rotundifolia) were collected from mature individuals at each study site. Seeds were placed in mesh seed bags and buried in experimental plots in the field. At each site, seed bags were deployed both in plots containing adult plants (occupied plots) and in control plots without adult individuals (unoccupied plots), allowing comparison of germination and fungal associations under different ecological conditions. Seed bags were retrieved annually over a three-year period following sowing. Upon retrieval, germination status and developmental stage of seedlings were recorded. Seedlings were categorized into developmental stages (germinated, elongated, and branched), and samples of germinated seedlings were collected for molecular analysis of associated fungal communities.
| Área de Estudo | The study was conducted on the island of Öland, Sweden, across multiple forest sites representing different habitat types, including old-growth coniferous forests dominated by Pinus sylvestris and mixed coniferous–deciduous forests. The sites were selected to represent suitable habitats for the target plant species and to allow comparison between locations with and without established adult individuals of the studied species. |
|---|---|
| Controle de Qualidade | Sequence data were subjected to multiple quality control steps to ensure reliability. Raw reads were filtered to remove low-quality sequences, chimeric sequences, and singletons. Only sequences passing quality thresholds were retained for further analysis. Operational taxonomic units (OTUs) were clustered at a 97 percent sequence similarity threshold. Taxonomic assignments were performed using reference databases, and assignments were accepted based on sequence similarity criteria. Negative controlsitem-0 were included during laboratory procedures to detect contamination, and non-target sequences were excluded from analyses where appropriate (Johansson et al. 2017). Re-annotation against UNITE database took place before publication of this dataset (2026-03-30). |
Descrição dos passos do método:
- DNA was extracted from germinated seedlings using a soil DNA extraction protocol. The internal transcribed spacer (ITS) region was amplified using PCR with primers targeting fungal ITS regions. Amplicons were normalized, pooled, and sequenced using 454 pyrosequencing technology. Sequence data were processed using bioinformatics pipelines that included demultiplexing, trimming, and clustering into OTUs. Representative sequences for each OTU were compared against reference databases (including UNITE and INSDC) for taxonomic identification. OTUs were assigned to taxonomic levels based on sequence similarity thresholds, and fungal taxa of interest, particularly ectomycorrhizal groups, were identified for further ecological analysis.
Metadados Adicionais
| Introduction | |
|---|---|
| Getting Started | |
| Propósito | |
| Identificadores alternativos | d19074ea-f1cf-42ba-9109-e99a5e6a50a5 |
| https://www.gbif.se/ipt/resource?r=pyrola-its2-2011-2012 |