Описание
Записи данных
Данные этого находка ресурса были опубликованы в виде Darwin Core Archive (DwC-A), который является стандартным форматом для обмена данными о биоразнообразии в виде набора из одной или нескольких таблиц. Основная таблица данных содержит 9 922 записей.
Также в наличии 2 таблиц с данными расширений. Записи расширений содержат дополнительную информацию об основной записи. Число записей в каждой таблице данных расширения показано ниже.
Данный экземпляр IPT архивирует данные и таким образом служит хранилищем данных. Данные и метаданные ресурсов доступны для скачивания в разделе Загрузки. В таблице версий перечислены другие версии ресурса, которые были доступны публично, что позволяет отслеживать изменения, внесенные в ресурс с течением времени.
Версии
В таблице ниже указаны только опубликованные версии ресурса, которые доступны для свободного скачивания.
Как оформить ссылку
Исследователи должны дать ссылку на эту работу следующим образом:
Johansson V (2026). Fungal ITS2 data from Pyroleae and Monotropa hypopitys germination study. Version 1.1. Stockholm University. Occurrence dataset. https://www.gbif.se/ipt/resource?r=pyrola-its2-2011-2012&v=1.1
Права
Исследователи должны соблюдать следующие права:
Публикующей организацией и владельцем прав на данную работу является Stockholm University. Эта работа находится под лицензией Creative Commons Attribution (CC-BY 4.0).
Регистрация в GBIF
Этот ресурс был зарегистрирован в GBIF, ему был присвоен следующий UUID: d19074ea-f1cf-42ba-9109-e99a5e6a50a5. Stockholm University отвечает за публикацию этого ресурса, и зарегистрирован в GBIF как издатель данных при оподдержке GBIF Sweden.
Ключевые слова
Occurrence; ITS; metabarcoding; fungal communities; ectomycorrhizal fungi; mycoheterotrophy; seedling development; Pyroleae; Monotropa hypopitys; dust seeds; Sweden
Контакты
- Metadata Provider ●
- Originator ●
- Point Of Contact
- GBIF Sweden Node Manager
- Box 50007
- +468-51954036
https://orcid.org/https://orcid.org/0000-0002-3028-9947
- Principal Investigator
- Author
- Author
- Author
Географический охват
Sampling was conducted on the island of Öland, Sweden, across multiple forest sites including coniferous and mixed forests.
| Ограничивающие координаты | Юг Запад [56,438, 16,387], Север Восток [57,353, 17,093] |
|---|
Таксономический охват
N/A
| Kingdom | Fungi |
|---|---|
| Phylum | Mortierellomycota, Basidiobolomycota, Entorrhizomycota, Mucoromycota, Ascomycota, Zoopagomycota, Rozellomycota, Basidiomycota, Chytridiomycota |
| Class | Geminibasidiomycetes, Rhizophydiomycetes, Rozellomycota_cls_Incertae_sedis, Tremellomycetes, GS27, Atractiellomycetes, Spizellomycetes, Mortierellomycetes, Microbotryomycetes, Basidiobolomycota_cls_Incertae_sedis, Malasseziomycetes, Pezizomycotina_cls_Incertae_sedis, Dothideomycetes, Candelariomycetes, Eurotiomycetes, Entorrhizomycetes, Umbelopsidomycetes, Exobasidiomycetes, Sordariomycetes, Saccharomycetes, Lobulomycetes, Geoglossomycetes, Mucoromycetes, Pucciniomycetes, Rhizophlyctidomycetes, Archaeorhizomycetes, Arthoniomycetes, Agaricomycetes, Cystobasidiomycetes, Ascomycota_cls_Incertae_sedis, Rozellomycotina_cls_Incertae_sedis, Zoopagomycetes, Pezizomycetes, Basidiobolomycetes, Leotiomycetes, Orbiliomycetes |
| Order | Leucosporidiales, Pezizomycotina_ord_Incertae_sedis, Microthyriales, Filobasidiales, Myrmecridiales, Agaricomycetes_ord_Incertae_sedis, Eurotiomycetidae_ord_Incertae_sedis, Lichenostigmatales, Trichosphaeriales, Microbotryomycetes_ord_Incertae_sedis, Auriculariales, Sordariales, Leotiomycetes_ord_Incertae_sedis, Rhizophlyctidales, Cladosporiales, Mytilinidiales, Orbiliales, Onygenales, Verrucariales, Helicobasidiales, Exobasidiomycetes_ord_Incertae_sedis, Malasseziales, Tremellodendropsidales, Microascales, Cystofilobasidiales, Trichosporonales, Sebacinales, Dothideomycetes_ord_Incertae_sedis, Geminibasidiales, Corticiales, Geoglossales, Acrospermales, Mortierellales, Pseudodactylariales, Rhytismatales, Asterinales, Helotiales, Mucorales, Chaetosphaeriales, Agaricales, GS11, Cephalothecales, Atheliales, Trechisporales, Entorrhizales, Annulatascales, Rozellomycota_ord_Incertae_sedis, Basidiobolomycota_ord_Incertae_sedis, Candelariales, Pleosporales, Umbelopsidales, Pezizales, Glomerellales, Kriegeriales, Spizellomycetales, Polyporales, Sporidiobolales, Zoopagales, Mycosphaerellales, Eurotiales, Capnodiales, Patellariales, GS27_ord_Incertae_sedis, Hymenochaetales, Sclerococcales, Xylariales, Russulales, Hypocreales, Atractiellales, Botryosphaeriales, Tremellales, Chaetothyriales, Venturiales, Exobasidiales, Thelebolales, Tubeufiales, Magnaporthales, Thelephorales, Rhizophydiales, Leotiales, Gloniales, Lobulomycetales, Saccharomycetales, Boletales, Ascomycota_ord_Incertae_sedis, Archaeorhizomycetales, Coniochaetales, Cantharellales, Basidiobolales, Cystobasidiales, Phacidiales, Chaetomellales, Dothideales, Amphisphaeriales |
Методы сбора
Seeds of five plant species (Chimaphila umbellata, Moneses uniflora, Monotropa hypopitys, Pyrola chlorantha, and Pyrola rotundifolia) were collected from mature individuals at each study site. Seeds were placed in mesh seed bags and buried in experimental plots in the field. At each site, seed bags were deployed both in plots containing adult plants (occupied plots) and in control plots without adult individuals (unoccupied plots), allowing comparison of germination and fungal associations under different ecological conditions. Seed bags were retrieved annually over a three-year period following sowing. Upon retrieval, germination status and developmental stage of seedlings were recorded. Seedlings were categorized into developmental stages (germinated, elongated, and branched), and samples of germinated seedlings were collected for molecular analysis of associated fungal communities.
| Охват исследования | The study was conducted on the island of Öland, Sweden, across multiple forest sites representing different habitat types, including old-growth coniferous forests dominated by Pinus sylvestris and mixed coniferous–deciduous forests. The sites were selected to represent suitable habitats for the target plant species and to allow comparison between locations with and without established adult individuals of the studied species. |
|---|---|
| Контроль качества | Sequence data were subjected to multiple quality control steps to ensure reliability. Raw reads were filtered to remove low-quality sequences, chimeric sequences, and singletons. Only sequences passing quality thresholds were retained for further analysis. Operational taxonomic units (OTUs) were clustered at a 97 percent sequence similarity threshold. Taxonomic assignments were performed using reference databases, and assignments were accepted based on sequence similarity criteria. Negative controlsitem-0 were included during laboratory procedures to detect contamination, and non-target sequences were excluded from analyses where appropriate (Johansson et al. 2017). Re-annotation against UNITE database took place before publication of this dataset (2026-03-30). |
Описание этапа методики:
- DNA was extracted from germinated seedlings using a soil DNA extraction protocol. The internal transcribed spacer (ITS) region was amplified using PCR with primers targeting fungal ITS regions. Amplicons were normalized, pooled, and sequenced using 454 pyrosequencing technology. Sequence data were processed using bioinformatics pipelines that included demultiplexing, trimming, and clustering into OTUs. Representative sequences for each OTU were compared against reference databases (including UNITE and INSDC) for taxonomic identification. OTUs were assigned to taxonomic levels based on sequence similarity thresholds, and fungal taxa of interest, particularly ectomycorrhizal groups, were identified for further ecological analysis.
Дополнительные метаданные
| Введение | |
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| Приступая к работе | |
| Цель | |
| Альтернативные идентификаторы | d19074ea-f1cf-42ba-9109-e99a5e6a50a5 |
| https://www.gbif.se/ipt/resource?r=pyrola-its2-2011-2012 |